Development and Application of New Technologies for Comprehensive and Quantitative High Throughput Microbial Proteomics
نویسندگان
چکیده
With recent advances in whole genome sequencing for an increasing number of organisms, biological research is increasingly incorporating higher-level “systems” perspectives and approaches. Biology is transitioning from a largely qualitative descriptive science to a quantitative, ultimately predictive science. Key to supporting advances in microbial and other biological research at the heart of the DOE Genomics: GTL program is the ability to quantitatively measure the array of proteins (i.e., the proteome) in various biological systems under many different conditions. The challenges associated with making useful comprehensive proteomic measurements include identifying and quantifying large sets of proteins that have relative abundances spanning many orders of magnitude, which vary broadly in chemical and physical properties, have transient and low levels of modifications, and are subject to endogenous proteolytic processing. Ultimately, such measurements, and the resulting understandings of biochemical processes are expected to enable development of predictive computational models that could profoundly affect environmental clean-up and energy production by, for example, providing a more solid basis for mitigating the impacts of energy-production-related activities on the environment and human health.
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